Table 6 Eco-scale penalty points of the developed TLC method for simultaneous determination of propranolol and methimazole in spiked human plasma

Acetone

1 (3 ~ < 10 mL)

2 (1 pictograms, danger)

2

Ethyl acetate

2 (27 ~ 10–100 mL)

4 (2 pictograms, danger)

8

NH₃ solution

1 (0.05 ~ < 10 mL)

6 (3 pictograms, danger)

6

The Instruments

 Energy used

1 (LC < 1.5 kWh per sample)

 Occupational hazard

0

 Waste^a

3 (1.5 ~ 1–10 mL)

 Total penalty points

Σ 20

 Analytical Eco-scale score

80

RGB model