Thyrotoxicosis antidote assay along with concurrent medication; chromatographic and environmental issues

BMC Chemistry

Table 2 Intra and inter-assay precision and accuracy of LLOQ, LQC, MQC, and HQC of propranolol and methimazole in spiked human plasma samples

Component	Concentration (µg/band)^a		Intra-day			Inter-day
Component	Concentration (µg/band)^a		Recovery %	RSD %	Bias %^b	Recovery %	RSD %	Bias %^b
PRP	LLOQ	0.06	103.61	2.090	3.61	102.83	2.971	2.83
	LQC	0.2	104.44	3.990	4.44	98.72	4.018	− 1.28
	MQC	0.4	100.39	1.890	0.39	102.54	3.111	2.54
	HQC	1.0	103.30	1.040	3.30	99.28	2.882	− 0.82
MTZ	LLOQ	0.1	96.01	1.798	− 3.99	102.24	3.178	2.24
	LQC	0.3	96.65	2.820	− 3.35	104.85	4.281	4.85
	MQC	0.6	98.35	1.987	− 1.65	102.07	2.753	2.07
	HQC	1.0	100.72	3.059	0.72	97.21	4.071	− 2.79

^a Average of 3 experiments
^b Bias = [(measured concentration—nominal concentration)/nominal concentration] × 100

ISSN: 2661-801X