Fig. 3
2D chromatogram of a blank human plasma, b plasma and 0.5 µg/band of the internal standard hydrocortisone acetate, c spiked human plasma with 0.8 µg/band of propranolol, 1.0 µg/band of methimazole and 0.5 µg/band of the internal standard hydrocortisone acetate, using ethyl acetate: acetone: 33% NH3 solution (9: 1: 0.05, by volume) as a developing system at 254 nm