Table 1 Chromatographic parameters for CLR and MOX analysis using the RP-HPLC chromatographic method

Stationary phase

Supelcosil C18 (4.6-mm×15-cm, 5µ)

Mobile phase

Acetonitrile: Methanol: Water: Acetic acid (56:36:7.5:0.50, by volume)

Detector

UV (254 nm)

Pumping sytem

Isocratic

Temperature

Room temperature at 25 ⁰C

The Injected volume

10 µL

Flow rate

2.0 mL min⁻¹

Total run time

9 min

Retention times

CLR: 1.43 min

MOX: 6.05 min

Acidic degradation products (3.50 min)

Basic and oxidative degradation products (3.10 min)