Table 3 Application of the developed fluorometric and colorimetric methods and the comparison method for Estimation of VER in Raw materials
Parameters | Spectrofluorometric method | Comparison method (n = 3) [10] | Spectrophotometric method | Comparison method (n = 3) [10] | ||
|---|---|---|---|---|---|---|
VER (pure form) | Amount taken (µg mL− 1) | % Recovery ± SD | Amount taken (µg mL− 1) | % Recovery ± SD | ||
0.05 | 99.89 ± 1.11 | 99.76 ± 0.85 | 0.5 | 101.65 ± 0.99 | 100.32 ± 1.35 | |
0.10 | 100.76 ± 1.76 | 100.42 ± 1.01 | 2.5 | 100.98 ± 0.75 | 100.68 ± 1.02 | |
0.20 | 100.64 ± 0.86 | 99.66 ± 0.95 | 4.5 | 99.75 ± 0.54 | 99.99 ± 0.97 | |
0.30 | 99.07 ± 0.97 | 6.5 | 100.87 ± 1.65 | |||
0.40 | 100.26 ± 1.77 | 8.5 | 99.54 ± 1.53 | |||
0.50 | 99.75 ± 1.74 | 10.0 | 98.59 ± 1.94 | |||
X− ± SD | 100.06 ± 1.37 | 99.95 ± 0.94 | 100.23 ± 1.23 | 100.33 ± 1.11 | ||
t -test | 0.865 | 0.457 | ||||
F - value | 1.457 | 1.108 | ||||