Utility of green chemistry for spectrofluorometric and spectrophotometric analysis of vericiguat via reaction with erythrocin B

Table 1 Validation data of VER by the proposed methods

Parameter	Spectrofluorometric method	Spectrophotometric method
Concentration range (µg mL^− 1)	0.05–0.5	0.5–10.0
Detection limit (µg mL^− 1)	0.012	0.131
Quantitation limit (µg mL^− 1)	0.036	0.412
Determination coefficient (r²)	0.9996	0.9999
Slope	1131	0.0578
Intercept	47.83	0.0729
S^y/x	1.8358	0.0061
S_a	12.467	0.0075
S_b	17.796	0.0083
% Error	0.764	0.675
% RSD	1.964	1.432
Mean found (%) ± SD	100.76 ± 0.987	99.89 ± 1.01

Note: S_a, standard deviation of the intercept of regression line; S_b, standard deviation of the slope of regression line; S_y/x, standard deviation of the residuals

ISSN: 2661-801X