Table 1 Analytical performance data for the proposed method

Wavelength (nm)

390

399

Linearity range (ng/mL)

5 ─ 150

10 ─ 600

Slope ± SD

0.0340 ± 0.0006

0.0039 ± 0.0001

Intercept ± SD

0.9964 ± 0.0199

0.4763 ± 0.0095

LOD (ng/mL)

1.415

3.021

LOQ (ng/mL)

4.287

9.156

Coefficient of determination (r²)

0.9996

0.9995

Accuracy (R%) ^a

99.03

98.94

Precision (RSD%)^b

- Repeatability

- Intermediate precision

1.290

1.151

0.870

1.341

Robustness (R^c%± RSD %)

- Δλ (± 1 nm)

- pH (± 0.1)

- Britton-Robinson buffer volume (± 0.1 mL)

99.87 ± 1.102

98.43 ± 0.497

100.72 ± 0.691

100.24 ± 1.351

99.07 ± 1.093

99.61 ± 1.572

Molecular weight

602.6 g/mol

236.31 g/mol

Solubility

Soluble in Ethanol and methanol

Soluble in Ethanol, DMSO, and dimethyl formamide (DMF)

pKa (Strongest Acidic)

10.23

14

pKa (Strongest Basic)

0.65

7.65