Parameters | TLC -Densitometric method | HPLC | Reference value [39] | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
IBU | PAP | PAR | PNP | CHZ | PCA | PAP | PAR | PNP | IBU | CHZ | PCA | ||
Rf/tR | 0.12 | 0.38 | 0.48 | 0.55 | 0.62 | 0.68 | 3.7 | 5.5 | 6.9 | 8.3 | 9.6 | 11.0 | – |
Resolution (Rs)a | – | 6.56 | 2.94 | 2.87 | 2.05 | 2.47 | – | 4.5 | 4.7 | 8.2 | 7.2 | 5.2 | Rs > 2 |
Selectivity factor (α)a | – | 4.49 | 1.50 | 1.32 | 1.33 | 1.30 | – | 1.14 | 1.31 | 1.34 | 1.19 | 1.16 | α > 1 |
Tailing factor (T) | 0.94 | 1.06 | 0.95 | 1.07 | 1.06 | 1.08 | 0.8 | 1.0 | 1.2 | 1.1 | 1.0 | 1.1 | T = 0.8–1.2 |
Capacity factor (K’) | 7.33 | 1.63 | 1.08 | 0.82 | 0.61 | 0.47 | 2.1 | 3.6 | 4.7 | 5.9 | 7.0 | 8.1 | 1 < K’ < 10 |
Column efficiency (N) | – | – | – | – | – | – | 2156. 4 | 3348.9 | 25,114.2 | 37,683.8 | 26,916.5 | 22,501.5 | ˃ 2000 |
Height equivalent to theoretical plate (mm) | – | – | – | – | – | – | 0.070 | 0.045 | 0.006 | 0.004 | 0.006 | 0.007 | The smaller the value, the higher the efficiency |