Skip to main content

Table 1 System suitability parameters of TLC-densitometric and HPLC methods

From: Impurity profiling of paracetamol toxic impurities in pharmaceutical combination with ibuprofen and chlorzoxazone using HPLC and TLC densitometric methods

Parameters

TLC -Densitometric method

HPLC

Reference value [39]

IBU

PAP

PAR

PNP

CHZ

PCA

PAP

PAR

PNP

IBU

CHZ

PCA

Rf/tR

0.12

0.38

0.48

0.55

0.62

0.68

3.7

5.5

6.9

8.3

9.6

11.0

Resolution (Rs)a

6.56

2.94

2.87

2.05

2.47

4.5

4.7

8.2

7.2

5.2

Rs > 2

Selectivity factor (α)a

4.49

1.50

1.32

1.33

1.30

1.14

1.31

1.34

1.19

1.16

α > 1

Tailing factor (T)

0.94

1.06

0.95

1.07

1.06

1.08

0.8

1.0

1.2

1.1

1.0

1.1

T = 0.8–1.2

Capacity factor (K’)

7.33

1.63

1.08

0.82

0.61

0.47

2.1

3.6

4.7

5.9

7.0

8.1

1 < K’ < 10

Column efficiency (N)

2156. 4

3348.9

25,114.2

37,683.8

26,916.5

22,501.5

˃ 2000

Height equivalent to theoretical plate (mm)

0.070

0.045

0.006

0.004

0.006

0.007

The smaller the value, the higher the efficiency

  1. a Chromatographic resolution and selectivity factor are determined between each peak and the one preceding it