Development and validation of a novel fluorometric approach utilizing flow injection analysis for the measurement of vilazodone: application to dosage form and spiked human plasma

Table 4 Robustness study of the proposed method for the determination of 200 ng mL^-1 VZN (n = 3)

Parameter		Recovery͙͙ (%)	Standard deviation	RSD (%)
Optimum condition		98.95	1.15	1.16
Flow rate	0.4	101.51	0.92	0.91
Flow rate	0.6	99.83	1.34	1.34
Percentage of acetonitrile (%)	58	98.72	1.44	1.45
Percentage of acetonitrile (%)	62	100.59	0.74	0.74
pH	4.9	100.68	1.38	1.37
pH	5.1	101.59	0.76	0.75
λ excitation (nm)	239	99.09	0.84	0.85
λ excitation (nm)	243	99.25	0.94	0.95
λ emission (nm)	484	99.55	1.07	1.08
λ emission (nm)	488	98.48	1.52	1.55

ISSN: 2661-801X