Table 1 The central composite design and the observed values are used to optimize the RP-UPLC method for Ensifentrine optimization
Run No | Run | A: Flow rate (Factor 1, mL/min) | B: Organic Phase-Mobile phase (Factor 2, %) | C: Temperature (Factor 3, oC) | Response 1 (RT-Retention Time, min) | Response 2 (NTP-Number of Theoretical Plate, min) | Response 3(TF-Tailing Factor, min) |
|---|---|---|---|---|---|---|---|
1 | 11 | 0.27 | 25 | 27 | 1.266 | 4176.2 | 1.1 |
2 | 8 | 0.33 | 25 | 27 | 1.056 | 2531.1 | 1.03 |
3 | 2 | 0.27 | 35 | 27 | 1.291 | 4140.1 | 1.1 |
4 | 16 | 0.33 | 35 | 27 | 1.078 | 2588.5 | 1.1 |
5 | 19 | 0.27 | 25 | 33 | 1.189 | 2874.1 | 1.1 |
6 | 6 | 0.33 | 25 | 33 | 1.001 | 2455 | 1 |
7 | 17 | 0.27 | 35 | 33 | 1.215 | 2980.9 | 1.11 |
8 | 9 | 0.33 | 35 | 33 | 1.028 | 2470.2 | 1.1 |
9 | 12 | 0.249546 | 30 | 30 | 1.328 | 4254.8 | 1.1 |
10 | 7 | 0.350454 | 30 | 30 | 0.979 | 2380 | 1.04 |
11 | 10 | 0.3 | 21.591 | 30 | 1.118 | 2791.8 | 1.02 |
12 | 4 | 0.3 | 38.409 | 30 | 1.163 | 2797.7 | 1.1 |
13 | 1 | 0.3 | 30 | 24.9546 | 1.197 | 3349.7 | 1.1 |
14 | 15 | 0.3 | 30 | 35.0454 | 1.063 | 2591.5 | 1.1 |
15 | 18 | 0.3 | 30 | 30 | 1.121 | 2697 | 1.08 |
16 | 14 | 0.3 | 30 | 30 | 1.123 | 2693 | 1.08 |
17 | 5 | 0.3 | 30 | 30 | 1.124 | 2709 | 1.07 |
18 | 3 | 0.3 | 30 | 30 | 1.124 | 2724 | 1.08 |
19 | 20 | 0.3 | 30 | 30 | 1.134 | 2804 | 1.08 |
20 | 13 | 0.3 | 30 | 30 | 1.135 | 2791 | 1.08 |