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Table 4 Linear regression data for the calibration curve of Nys and TA along with their LOD and LOQ data (mean ± SD, n = 6)

From: Ultra-performance liquid chromatography method for quantitative analysis of nystatin and triamcinolone acetonide in topical creams after in vitro release using franz diffusion cell

Parameters

TA

Nys

Linearity range

0.65–31.93 µg/mL

17.67–863.27 IU/mL

Regression equation

y = 39942x + 1753.8

y = 6198.9x + 12,007

r2

1.0000

1.0000

Slope

39,942 ± 1012

6198.9 ± 103.0

Intercept

1753.8 ± 21.0

12,007 ± 411

Standard error of slope

584.29

59.46

Standard error of intercept

12.12

237.29

95% confidence interval of slope

36,727–41,756

5943–6454

95% confidence interval of intercept

1701–1805

10,986–13,028

LOD

0.12 ± 0.01 µg/mL

0.40 ± 0.02 IU/mL

LOQ

0.41 ± 0.03 µg/mL

1.33 ± 0.06 IU/mL