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Table 7 Green analytical procedure index parameters description for proposed and reported [6] methods for determination of vit. C and vit. A

From: Eco-friendly RP-HPLC method for simultaneous determination of water-soluble and fat-soluble vitamins in nano-formula and pharmaceutical dosage forms

Category

Proposed Method

Reported Method

Type

Color

Type

Color

Sample preparation

Collection(1)

In line

Green

In line

Green

Preservation (2)

None

Green

None

Green

Transport (3)

None

Green

None

Green

Storage (4)

None

Green

None

Green

Type of method: direct or indirect (5)

Simple procedure (filtration)

Yellow

Simple procedure (filtration)

Yellow

Scale of extraction (6)

None

Green

None

Green

Solvent/reagent used (7)

Green solvent

Green

Green solvent

Green

Additional treatment (8)

None

Green

None

Green

Reagent and solvents

Amount (9)

< 10 ml

Green

10–100 ml

Yellow

Health hazard (10)

Moderate toxicity

Yellow

Moderate toxicity

Yellow

Safety hazard (11)

Flammable

Yellow

Flammable

Yellow

Instrument

Energy (12)

≤ 1.5 kWh per sample

Yellow

≤ 1.5 kWh per sample

Yellow

Occupational hazard (13)

None

Green

None

Green

Waste (14)

< 10 mL

Yellow

> 10

Red

Waste treatment (15)

Passivation

Yellow

Passivation

Yellow

Additional Mark: Quantification

Circle in the middle of GAPI

Yes

Qualitative and quantitative method.

Yes

Qualitative and quantitative method.