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Table 3 Statistical analysis of the proposed method and the reported method [6] of vit. C and vit. A in the pure powder form

From: Eco-friendly RP-HPLC method for simultaneous determination of water-soluble and fat-soluble vitamins in nano-formula and pharmaceutical dosage forms

Drug

Method

Mean ± RSD

STDEV

VARIANCE

N

t-test (2.262) a

F-ratio (6.26) a

Vit. C

HPLC

99.49% ± 1.58

1.57

2.46

6

0.99

1.46

Reported*

100.31% ± 1.30

1.30

1.69

6

  

Vit. A

HPLC

100.26% ± 1.68

1.68

2.83

6

0.04

1.99

Reported*

100.30% ± 1.19

1.19

1.42

6

  
  1. (a) Theoretical value for t-test and F-ratio for P = 0.05
  2. * HPLC method using octadecylsilane column, mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 and methanol in gradient elution at the flow rate 0.7 ml min− 1. And detection at 280 nm
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BMC Chemistry

ISSN: 2661-801X

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