Table 1 Assay validation parameters and regression equations for determination of vit. C and vit. A by the proposed chromatographic method

Linearity

1–60 µg.mL^− 1

0.5–30 IU.mL^− 1

Regression equation

y = 34486x– 26,475

y = 23988x + 2052.4

Correlation coefficient (r)

0.9997

0.9999

Accuracy (Mean ± RSD)

99.49% ± 1.58

100.26% ± 1.86

LOD

0.3 µg.mL^− 1

0.15 IU.mL^− 1

LOQ

1 µg.mL^− 1

0.5 IU.mL^− 1

Repeatability ⁽¹⁾

101.26% ± 0.91

98.27% ± 0.63

Reproducibility ⁽²⁾

101.41% ± 0.69

98.66% ± 0.89