Development of UV-Chemometric techniques for resolving the overlapped spectra of aspirin, caffeine and orphenadrine citrate in their combined pharmaceutical dosage form

Table 3 Results obtained from PLS models for determination of ASP, CAF & ORP in calibration and validation sets

Spectra order		Zero			First derivative			Second derivative			Ratio spectra			Ratio derivative			Ratio 2^nd derivative
PLS	Parameter	ASP	CAF	ORP	ASP	CAF	ORP	ASP	CAF	ORP	ASP	CAF	ORP	ASP	CAF	ORP	ASP	CAF	ORP
Calibration set	Mean	99.78	100.32	99.51	99.81	100.00	100.20	99.97	100.31	100.56	98.02	103.50	102.55	98.72	104.04	103.45	99.63	106.17	105.22
	RMSEP	0.2304	0.2271	0.2571	0.2303	0.1699	0.2356	0.3074	0.3154	0.3228	3.4975	2.4447	3.1148	3.6164	2.6107	3.1372	2.9212	2.7890	2.9719
	PRESS	0.9023	0.8771	1.1237	0.9019	0.4905	0.9432	1.6063	1.6910	1.7711	207.9551	101.5981	164.9332	222.3275	115.8640	167.3122	145.0681	132.2380	150.1522
Validation set	Mean	100.58	99.44	101.18	100.54	100.02	99.80	100.33	99.60	99.12	117.87	104.36	105.69	116.20	102.68	104.09	118.97	104.83	107.89
	RMSEP	0.1504	0.1503	0.3161	0.1743	0.1324	0.2590	0.2728	0.2459	0.2179	3.3262	4.1954	1.8864	2.9421	3.4651	1.7904	2.8550	2.6931	2.4248
	PRESS	0.1808	0.1806	0.7996	0.2431	0.1402	0.5368	0.5954	0.4837	0.3798	88.5084	140.8140	28.4672	69.2457	96.0542	25.6439	65.2098	58.0221	47.0382

ISSN: 2661-801X