Table 1 Experimental design of 25 calibration mixtures formulated using a five-level, five-factor approach, and an additional 13 validation mixtures generated by KSC for chemometric models’ validation
Mix no | Calibration set (µg/mL) | |||
|---|---|---|---|---|
MOM | OLO | MTS | DAP | |
1. | 4 | 46.5 | 2.5 | 3 |
2. | 4 | 6.5 | 0.5 | 5 |
3. | 1 | 6.5 | 4.5 | 2 |
4. | 1 | 86.5 | 1.5 | 5 |
5. | 7 | 26.5 | 4.5 | 3 |
6. | 2.5 | 86.5 | 2.5 | 2 |
7. | 7 | 46.5 | 1.5 | 2 |
8. | 4 | 26.5 | 1.5 | 4 |
9. | 2.5 | 26.5 | 3.5 | 5 |
10. | 2.5 | 66.5 | 4.5 | 4 |
11. | 5.5 | 86.5 | 3.5 | 3 |
12. | 7 | 66.5 | 2.5 | 5 |
13. | 5.5 | 46.5 | 4.5 | 5 |
14. | 4 | 86.5 | 4.5 | 1 |
15. | 7 | 86.5 | 0.5 | 4 |
16. | 7 | 6.5 | 3.5 | 1 |
17. | 1 | 66.5 | 0.5 | 3 |
18. | 5.5 | 6.5 | 2.5 | 4 |
19. | 1 | 46.5 | 3.5 | 4 |
20. | 4 | 66.5 | 3.5 | 2 |
21. | 5.5 | 66.5 | 1.5 | 1 |
22. | 5.5 | 26.5 | 0.5 | 2 |
23. | 2.5 | 6.5 | 1.5 | 3 |
24. | 1 | 26.5 | 2.5 | 1 |
25. | 2.5 | 46.5 | 0.5 | 1 |
Mix no | Validation set (µg/mL) | |||
|---|---|---|---|---|
MOM | OLO | MTS | DAP | |
1. | 7 | 29 | 4 | 4.5 |
2. | 3 | 19 | 3 | 4 |
3. | 3 | 48 | 2 | 3 |
4. | 6 | 76 | 3 | 4.5 |
5. | 3 | 85 | 3.5 | 2 |
6. | 4 | 75 | 3 | 2.5 |
7. | 5 | 18 | 2 | 1 |
8. | 2 | 86 | 2 | 1 |
9. | 4 | 41 | 1 | 4.5 |
10. | 2 | 55 | 4 | 3 |
11. | 2 | 8 | 1 | 4 |
12. | 1 | 72 | 4 | 3.5 |
13. | 6 | 33 | 1 | 2 |