Fig. 4
From: Gene-activation of surface-modified 3D printed calcium phosphate scaffolds
A Composite micrographs (fluorescence and brightfield) of HEK 293 T cells treated with either water (Untreated), polyplex solution (Polyplexes), centrifuged polyplex solution (Polyplex Supernatant), CPC powder mixed with polyplex solution (CPC/Poly Mixture), or the supernatant from the centrifugation of the previous mixture (CPC/Poly Supernatant). Green coloration indicates fluorescence from EGFP. All scalebars represent 400 μm in length. B Transfection efficiency and C log transformed mean fluorescence intensity for HEK 293 T cells treated as described above (n = 3). Cells were incubated for 72 h after transfection. Bars represent mean + SD where ****p < 0.0001