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Table 8 Penalty points calculation for the greenness evaluation of the present method

From: Facile nucleophilic substitution approach for the spectrofluorimetric assay of natamycin based on diarylpyrrolone formation, evaluation of method greenness

Item

Parameter

Word sign

PP score

Technique

Spectrofluorimetry

LSH

0

Reagent(s)

Fluorescamine

MSH *

1

Amount of reagent

> 10 mL

 

1

Solvent(s)

Acetone (for flurescamine)

Ethanol (for dilution)

MSH

LSH

4

1

Heating

No heating

 

0

Temperature

Ambient temperature

 

0

pH

7.6

 

0

Cooling

No cooling

 

0

Energy (kWh per sample)

> 1.0

 

0

Waste

1–10 mL

 

3

Occupational hazards

  

0

(TPPs)

  

10

Eco-scale total score

= 100 – TPP

 

90

  1. * MSH is an abbreviation for the More severe hazard, LSH for the Less severe hazard, and TPPs for the Total penalty points