Assessment of greenness, blueness, and whiteness profiles of a validated HPLC-DAD method for quantitation of Donepezil HCl and Curcumin in their laboratory prepared co-formulated nanoliposomes

Table 1 HPLC-DAD gradient elution program for analysis of DPZ and CUR

Time (min.)	Solvent A% (Buffer)	Solvent B% (Ethanol)	Flow rate (ml/ min.)
0	20	80	1.5
1	30	70	1.5
2	40	60	1.5
3	50	50	1.5
4	60	40	1.5
5	70	30	1.5
6	80	20	1.5
8	80	20	1.5

ISSN: 2661-801X

Time (min.)	Solvent A% (Buffer)	Solvent B% (Ethanol)	Flow rate (ml/ min.)
0	20	80	1.5
1	30	70	1.5
2	40	60	1.5
3	50	50	1.5
4	60	40	1.5
5	70	30	1.5
6	80	20	1.5
8	80	20	1.5

Time (min.)	Solvent A% (Buffer)	Solvent B% (Ethanol)	Flow rate (ml/ min.)
0	20	80	1.5
1	30	70	1.5
2	40	60	1.5
3	50	50	1.5
4	60	40	1.5
5	70	30	1.5
6	80	20	1.5
8	80	20	1.5

Time (min.)	Solvent A% (Buffer)	Solvent B% (Ethanol)	Flow rate (ml/ min.)
0	20	80	1.5
1	30	70	1.5
2	40	60	1.5
3	50	50	1.5
4	60	40	1.5
5	70	30	1.5
6	80	20	1.5
8	80	20	1.5