Establishment and validation of HPLC methods for the determination of folic acid and parabens antimicrobial agents on folic acid oral solution

Table 7 Folic acid content under different conditions

Conditions		Content (%)	Average (%)	RSD (%)
column temperatures	23℃	95.16	94.93	0.12
column temperatures	27℃	94.89
wavelength	278 nm	94.93
wavelength	282 nm	94.98
flow rates	1.0 mL/min	94.95
flow rates	1.4 mL/min	94.90
mobile phase ratio	0:100	94.92
mobile phase ratio	3:97	94.68
pH	3.8	94.84
pH	4.2	94.84
chromatographic columns	1	95.13
chromatographic columns	2	94.88

ISSN: 2661-801X