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Table 6 The robustness test determination of folic acid

From: Establishment and validation of HPLC methods for the determination of folic acid and parabens antimicrobial agents on folic acid oral solution

Folic acid

Retention time

Peak area

Measurement

(mg/mL)

Recovery (%)

1

2

1

2

Reference substance(23 ℃)

15.252

14.847

6,361,149

6,363,876

1.8848

95.16

Sample(23 ℃)

14.36

14.278

6,060,137

6,048,984

Reference substance (27 ℃)

12.471

12.515

6,363,861

6,359,493

1.8795

94.89

Sample(27 ℃)

12.616

12.699

6,033,907

6,039,087

Reference substance(278 nm)

13.856

13.938

6,270,745

6,270,016

1.8803

94.93

Sample (278 nm)

14.042

14.087

5,951,942

5,953,225

Reference substance (282 nm)

14.125

14.161

6,399,222

6,398,509

1.8813

94.98

Sample (282 nm)

14.125

14.161

6,399,222

6,398,509

Reference substance

(1.0 min/mL)

16.867

7,638,304

7,614,675

7,638,304

1.8806

94.95

Sample(1.0 min/mL)

16.033

15.882

7,240,513

7,241,705

Reference substance

(1.4 min/mL)

11.261

11.226

5,466,639

5,467,894

1.8797

94.90

Sample(1.4 min/mL)

11.195

11.178

5,189,692

5,187,392

Reference substance (0:100)

15.871

15.852

6,353,276

6,367,619

1.8801

94.92

Sample (0:100)

15.848

15.846

6,040,206

6,034,328

Reference substance (3:97)

9.921

9.904

6,408,386

6,382,839

1.8753

94.68

Sample (3:97)

9.886

9.876

6,055,843

6,054,912

Reference substance (pH3.8)

11.494

11.527

6,395,394

6,385,509

1.8786

94.84

Sample (pH3.8)

11.56

11.589

6,061,361

6,060,524

Reference substance (pH4.2)

14.200

13.918

6,457,083

6,454,290

1.8785

94.84

Sample (pH4.2)

13.798

13.779

6,122,651

6,122,238

Reference substance (1)

14.679

14.685

6,349,952

6,352,587

1.8842

95.13

Sample (1)

14.683

14.679

6,043,476

6,040,425

Reference substance (2)

12.036

12.008

6,405,586

6,401,608

1.8793

94.88

Sample (2)

12.028

12.124

6,078,475

6,073,160

  1. Recovery (%) = Actual measurement/ Actual weighing x 100