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Table 5 The recovery test determination of folic acid and antimicrobial agents

From: Establishment and validation of HPLC methods for the determination of folic acid and parabens antimicrobial agents on folic acid oral solution

  

Actual weighing

(mg)

Retention time

Peak area

Average

Actual measurement (mg)

Recovery

(%)

1

2

1

2

Folic acid

1

37.82

14.505

14.533

4,510,035

4,503,936

4,506,986

35.14

92.91

2

38.60

14.523

14.548

4,757,955

4,764,633

4,761,294

37.12

96.17

3

39.10

14.561

14.562

4,834,363

4,836,470

4,835,417

37.70

96.42

4

49.17

14.562

14.63

5,996,421

6,001,340

5,998,881

46.77

95.12

5

49.50

14.631

14.67

6,044,620

6,031,411

6,038,016

47.07

95.10

6

49.49

14.683

14.679

6,043,476

6,040,425

6,041,951

47.11

95.18

7

57.60

14.721

14.697

7,022,502

7,024,135

7,023,319

54.76

95.06

8

58.14

14.752

14.844

7,069,209

7,069,042

7,069,126

55.11

94.79

9

58.77

14.891

14.97

7,259,623

7,204,119

7,231,871

56.38

95.94

Average (%)

95.19

Methyl paraben

1

54.12

8.502

8.463

1,399,767

1,400,817

1,400,292

49.86

92.13

2

56.28

8.449

8.448

1,582,447

1,581,774

1,582,111

56.34

100.10

3

56.04

8.445

8.443

1,571,028

1,570,958

1,570,993

55.94

99.82

4

70.59

8.442

8.444

2,011,359

2,011,138

2,011,249

71.62

101.46

5

69.72

8.441

8.44

1,959,653

1,959,386

1,959,520

69.78

100.08

6

70.49

8.443

8.443

1,980,077

1,981,346

1,980,712

70.53

100.06

7

83.15

8.446

8.445

2,329,245

2,330,695

2,329,970

82.97

99.78

8

84.03

8.442

8.445

2,358,651

2,358,530

2,358,591

83.99

99.95

9

82.86

8.447

8.445

2,285,789

2,285,814

2,285,802

81.39

98.23

 

Average (%)

99.07

Ethyl paraben

1

34.02

14.34

14.253

817,394

817,877

817,636

31.89

93.74

2

35.85

14.214

14.211

924,092

923,607

923,850

36.03

100.51

3

35.97

14.199

14.197

937,799

937,533

937,666

36.57

101.67

4

45.32

14.201

14.204

1,184,285

1,184,017

1,184,151

46.18

101.91

5

44.52

14.201

14.201

1,144,022

1,143,735

1,143,879

44.61

100.21

6

44.98

14.208

14.205

1,155,373

1,156,175

1,155,774

45.08

100.22

7

53.09

14.211

14.209

1,359,673

1,360,620

1,360,147

53.05

99.92

8

53.21

14.205

14.21

1,377,458

1,377,665

1,377,562

53.73

100.97

9

52.41

14.214

14.21

1,334,992

1,334,944

1,334,968

52.07

99.34

 

Average (%)

99.83

Propyl paraben

1

10.62

27.718

27.52

244,091

244,390

244,241

9.94

93.61

2

11.21

27.434

27.418

275,519

275,845

275,682

11.22

100.10

3

11.15

27.384

27.389

273,543

272,928

273,236

11.12

99.75

4

14.23

27.395

27.402

351,571

351,448

351,510

14.31

100.55

5

14.03

27.401

27.408

343,007

342,462

342,735

13.95

99.44

6

14.08

27.418

27.411

343,570

343,574

343,572

13.99

99.33

7

16.65

27.425

27.414

405,737

406,081

405,909

16.52

99.23

8

16.81

27.415

27.425

411,125

411,081

411,103

16.73

99.55

9

16.18

27.433

27.419

398,241

398,131

398,186

16.21

100.17

 

Average (%)

99.08

  1. The low (Coding 1, 2, and 3), moderate(Coding 4, 5, and 6) and high(Coding 7, 8, and 9) concentrations were arranged in parallel at each level, and two needles were injected in parallel for each sample
  2. Recovery (%) = Actual measurement/ Actual weighing x 100
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BMC Chemistry

ISSN: 2661-801X

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