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Table 1 Analytical parameters for determination of REM, DEX and FVP by the proposed HPTLC method in spiked human plasma

From: A newly developed high-performance thin layer chromatographic method for determination of remdesivir, favipiravir and dexamethasone, in spiked human plasma: comparison with the published methods

Parameters

Spiked human plasma samples

REM

DEX

FVP

Calibration range

(µg/band)

0.1–5

µg/band

0.1–5

µg/band

0.2–15

µg/band

Slope**

3.8000

3.2101

0.9371

Intercept

0.6153

1.3009

0.5403

Analytical curves

Correlation coefficient

0.9999

0.9999

1

Accuracy (RSD %)*

96.32 ± 5.80

96.95 ± 5.01

99.01 ± 1.95

LLOQ (µg/band)

0.1

0.1

0.2

ULOQ (µg/band)

5

5

15

  1. *Average of three determinations
  2. **The linearity was achieved using the regression equation: A = aX2 + bX + C
  3. a: coefficient 1. b: coefficient 2
  4. A = peak area ratio (peak area of the analyte/peak area of IS) for spiked human plasma sample, X = concentration µg/ band. C = intercept
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BMC Chemistry

ISSN: 2661-801X

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