Table 1 Regression parameters for determination of VON and ASP in their dosage form
Parameter | HPLC | HPTLC | ||
|---|---|---|---|---|
VON | ASP | VON | ASP | |
Linearity range | 0.5–10 µg/mL | 1–100 µg/mL | 0.1–2 µg/band | 0.1–10 µg/band |
LOQ | 0.5 µg/mL | 1 µg/mL | 0.1 µg/band | 0.1 µg/band |
LOD | 0.17 µg/mL | 0.33 µg/mL | 0.03 µg/band | 0.03 µg/band |
Intercept, (a) | -43.29 | -44.58 | 944.69 | 703.56 |
Slope, (b) | 76.48 | 50.44 | 2098.15 | 84.07 |
Correlation coefficient, (r) | 0.9991 | 0.9998 | 0.9993 | 0.9992 |
Standard deviation of intercept, Sa | 9.00 | 28.86 | 54.26 | 9.99 |
Standard deviation of slope, Sb | 1.83 | 0.59 | 44.27 | 1.99 |
Standard deviation of residuals, Sy/x | 14.16 | 46.39 | 67.26 | 16.84 |
F | 1749.79 | 7391.92 | 2246.06 | 1788.46 |
Significance F | 3.01 × 10− 5 | 3.47 × 10− 6 | 2.07 × 10− 5 | 2.91 × 10− 5 |