Table 3 Linearity parameters for the absorbance and fluorescence mode detection

Excitation wavelength(nm)

280

225

280 with filter K320

225 with filter K320

R_f

0.35 ± 0.05

0.23 ± 0.05

0.35 ± 0.05

0.23 ± 0.05

Experimental estimated range (ng. band⁻¹)

25–200

50–500

25–200

50–500

Intercept (a) ± SD^*

−74.05 ± 3.67

89.13 ± 11.58

−510.66 ± 22.2

24.97 ± 11.66

Slope (b) ± SD*

12.06 ± 0.44

6.49 ± 0.19

28.36 ± 0.55

2.77 ± 0.05

r^**

0.9990

0.9987

0.9994

0.9998

r^2***

0.9982

0.9976

0.9987

0.9996

LOD (ng/band)

1.004

5.97

2.59

13.88

LOQ (ng/band)

3.04

18.08

7.86

42.05