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Table 2 Tested mobile phases and design matrix used for chromatographic method optimization with their experimentally obtained responses

From: Sustainable and efficient monitoring of tryptophan and tyrosine serum levels: a green HPTLC method as a biomarker for type 2 diabetes

Exp. number

Factor levels

Responses

ACN V

pH

Buffer V

E Ac V

Rf

N

Rs

Rf (Trp)

Rf (Tyr)

N (Trp)

N (Tyr)

X1

X2

X3

X4

Y1

Y2

Y3

Y4

Y5

1

7

6.4

0

2

0.08

0.05

261.2

148.4

0.42

2

6

6.4

0

2

0.23

0.17

751.0

504.5

0.84

3

5

6.4

0

2

0.27

0.18

881.6

534.2

1.25

4

4

6.4

0

2

0.19

0.12

620.4

356.1

0.98

5

6

11

0.3

2

0.17

0.05

555.1

148.4

1.67

6

5

7

1

2

0.25

0.2

816.3

593.5

0.625

7

5

8

1

2

0.29

0.2

946.9

593.5

1.25

8

4

9

1

2

0.28

0.18

914.3

534.2

1.39

9

6

9

1

2

0.38

0.31

1240.0

873.3

0.976

10

5

9

1

3

0.17

0.06

555.2

178.0

1.53

11

5

9

0.5

2

0.24

0.15

783.7

445.1

1.25

12

5

9

1

2

0.33

0.22

1077.6

652.9

1.5

13

5

9

2

2

0.35

0.23

1142.9

685.5

1.67

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BMC Chemistry

ISSN: 2661-801X

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