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Table 2 Dual wavelength ratio spectrum method according to International Conference for Harmonisation (ICH) guidelines and pharmaceutical application

From: Spectrophotometric determination of olanzapine, fluoxetine HCL and its impurity using univariate and chemometrics methods reinforced by latin hypercube sampling: Validation and eco-friendliness assessments

Parameters

OLA

FLU

Accuracy ± RSD%a

100.72 ± 0.252

99.309 ± 1.277

Regression equation

y = 0.0677x + 0.045

y = 0.0075x—0.002

Correlation coefficient R2

1

0.999

Range (μg/ml)

4–20

5–50

Intraday precision RSD%b

1.229

0.805

Intraday precision RSD%c

1.149

1.001

Robustness RSD%d

0.646

1.179

LOD (μg/ml)

0.143

0.661

LOQ (μg/ml)

0.432

2.002

Pharmaceuticale ± SD

99.457 ± 1.218

101.368 ± .575

  1. amean of five determinations
  2. bRepeatability (n = 9), a mean of three concentrations of OLA (8,10,15 µg/ml), FLU (10,20,35 µg/ml) repeated three times within the day (intra-daily)
  3. cThe inter-daily precision (n = 9), a mean of three concentrations of OLA (6,10,15 µg/ml), FLU (10,15,25 µg/ml) repeated three times on three successive days
  4. dRobustness (slight modification to the method) (n = 6), a mean of three concentrations of OLA (4,15 µg/ml), FLU (12.5, 20 µg/ml) repeated three times
  5. emean of three determinations
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BMC Chemistry

ISSN: 2661-801X

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