Table 4 Characteristic parameters for the regression equations of the proposed MEKC and HPLC methods for the determination of HCT, CPL, LSP, VAL, ATR, VIC, BSL, AML and CVL in rat plasma
Parameters | Â | HCT | CPL | LSP | VAL | ATR | VIC | BSL | AML | CVL |
|---|---|---|---|---|---|---|---|---|---|---|
Detection Wavelength (nm) | MEKC | 220 | 210 | 210 | 210 | 210 | 220 | 220 | 210 | 220 |
HPLC | ||||||||||
Linearity range (µg/mL) | MEKC | 50–1000 | 100–1000 |  | 50–2000 | 50–1000 | 10–1000 | 50–1000 | 50–1000 |  – |
HPLC | 1–100 |  − | 1–100 | 2–200 | 5–100 | 2–100 | 5–100 | 1–100 | 5–100 | |
LLOQ (µg/mL) | MEKC | 50 | 100 |  − | 50 | 50 | 50 | 50 | 50 |  – |
HPLC | 1 |  − | 1 | 2 | 5 | 2 | 5 | 1 | 5 | |
LOD (µg/mL) | MEKC | 25 | 50 |  − | 20 | 30 | 5 | 30 | 25 |  – |
HPLC | 0.40 |  − | 0.57 | 1.46 | 2.75 | 1.29 | 1.23 | 0.45 | 2.63 | |
Intercept | MEKC | − 2.03 × 10–1 | − 1.05 × 10–1 |  − | 1.50 × 10–1 | 4.35 × 10–2 | 5.76 × 10–2 | − 4.76 × 10–2 | − 5.77 × 10–2 |  – |
HPLC | 1.83 × 10–2 |  − | 8.31 × 10–3 | 2.81 × 10–2 | − 1.35 × 10–2 | 4.50 × 10–2 | 7.06 × 10–4 | 8.36 × 10–3 | 2.70 × 10–1 | |
Slope | MEKC | 1.55 × 10–2 | 7.35 × 10–3 |  − | 1.10 × 10–2 | 7.35 × 10–3 | 2.05 × 10–2 | 9.79 × 10–3 | 8.32 × 10–3 |  – |
HPLC | 1.89 × 10–1 |  − | 4.63 × 10–2 | 4.79 × 10–2 | 1.04 × 10–1 | 1.10 × 10–1 | 3.97 × 10–2 | 1.12 × 10–1 | 2.56 × 10–1 | |
Correlation coefficient | MEKC | 0.9996 | 0.9997 |  – | 0.9999 | 0.9998 | 0.9998 | 0.9999 | 0.9996 |  – |
HPLC | 0.9999 |  – | 0.9999 | 0.9999 | 0.9995 | 0.9998 | 0.9999 | 0.9999 | 0.9996 | |
aSa | MEKC | 9.40 × 10–2 | 4.49 × 10–2 |  – | 4.53 × 10–2 | 2.31 × 10–2 | 6.82 × 10–2 | 2.93 × 10–2 | 4.85 × 10–2 |  – |
HPLC | 2.51 × 10–2 |  – | 8.78 × 10–3 | 2.33 × 10–2 | 9.56 × 10–2 | 4.74 × 10–2 | 1.63 × 10–2 | 1.66 × 10–2 | 2.24 × 10–1 | |
bSb | MEKC | 2.06 × 10–4 | 9.13 × 10–5 |  – | 5.49 × 10–5 | 5.07 × 10–5 | 1.60 × 10–4 | 6.43 × 10–5 | 1.06 × 10–4 |  – |
HPLC | 5.82 × 10–4 |  – | 2.03 × 10–4 | 2.68 × 10–4 | 1.87 × 10–3 | 1.02 × 10–3 | 3.20 × 10–4 | 3.85 × 10–4 | 4.39 × 10–3 | |
cSy/x | MEKC | 1.64 × 10–1 | 6.66 × 10–2 |  – | 9.57 × 10–2 | 4.03 × 10–2 | 1.37 × 10–1 | 5.11 × 10–2 | 8.47 × 10–2 |  – |
HPLC | 5.20 × 10–2 |  – | 1.82 × 10–2 | 4.76 × 10–2 | 1.47 × 10–1 | 8.62 × 10–2 | 2.51 × 10–2 | 3.44 × 10–2 | 3.45 × 10–1 | |
dF | MEKC | 5664 | 6479 |  – | 39,766 | 20,996 | 16,495 | 23,161 | 6113 |  – |
HPLC | 105,525 |  – | 51,894 | 32,109 | 3099 | 11,752 | 15,401 | 84,110 | 3395 | |
Significance F | MEKC | 7.85 × 10–9 | 1.43 × 10–7 |  – | 1.07 × 10–12 | 2.97 × 10–10 | 1.50 × 10–11 | 2.32 × 10–10 | 6.49 × 10–9 |  – |
HPLC | 5.25 × 10–12 |  | 3.09 × 10–11 | 1.03 × 10–10 | 1.28 × 10–5 | 4.34 × 10–8 | 1.15 × 10–6 | 9.25 × 10–12 | 1.11 × 10–5 |