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Table 3 Within- run and between run accuracy and precision of ECT in plasma samples

From: Determination of the extremolyte ectoine in plasma and a pharmacokinetic study in rats by a validated and BAGI-evaluated UPLC-MS/MS method

Conc. (ng/mL)

Within-run

Between-run

n

Mean a ± SD

Accuracy (RE %)

Precision (CV %)

n

Mean b ± SD

Accuracy (RE %)

Precision (CV %)

1

6

0.97 ± 0.12

-3.00

12.51

18

0.95 ± 0.07

-5.11

7.41

5

6

4.81 ± 0.27

-3.80

5.79

18

4.87 ± 0.30

-2.60

6.29

350

6

337.53 ± 8.06

-3.56

2.39

18

337.76 ± 8.61

-3.49

2.55

750

6

733.74 ± 22.96

-2.16

3.13

18

730.25 ± 24.68

-2.63

3.38

  1. a Mean of six replicate observations at each concentration
  2. b Mean of 18 replicate observations over three different analytical runs