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Table 1 UPLC gradient elution system

From: Determination of the extremolyte ectoine in plasma and a pharmacokinetic study in rats by a validated and BAGI-evaluated UPLC-MS/MS method

Time (min)

0.05% formic acid (A)

Acetonitrile (B)

0.0

55

45

0.5

65

35

2.5

75

25

6.5

65

35

7.0

55

45

8.5

55

45