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Table 3 Statistical comparison of the results obtained by the proposed methods and the reported one [17]

From: An eco-friendly smartphone based HPTLC method versus conventional densitometric one for determination of Naltrexone and Bupropion

 

NAL

BUP

Value

Densitometry

ImageJ

Color Picker

Reporteda

Densitometry

ImageJ

Color Picker

Reporteda

Mean

100.18

100.98

100.37

100.08

100.11

99.68

100.49

99.86

SD

1.92

1.86

1.97

1.98

1.89

1.93

1.99

1.63

%RSD

1.92

1.84

1.96

1.98

1.89

1.94

1.98

1.63

Variance

3.69

3.46

3.88

3.92

3.57

3.73

3.96

2.66

N

7

7

5

5

7

7

5

5

t-testb

0.087

(2.228)

0.796 (2.228)

0.232 (2.306)

 

0.245

(2.228)

0.175 (2.228)

0.548 (2.306)

 

F Valueb

1.063

(4.530)

1.133 (4.530)

1.010 (6.390)

 

1.344

(4.530)

1.402 (4.530)

1.490 (6.390)

 

Linearity*

0.4–24

0.4–24

0.8–20

6.25–18.75

0.6–18

2–24

5–20

37.5–112.5

LOD*

0.1

0.1

0.1

NA

0.2

0.6

0.6

NA

LOQ*

0.3

0.3

0.8

NA

0.6

2

5

NA

  1. NA Not available
  2. aRP-HPLC method with mobile phase composed phosphate buffer (pH 3) and acetonitrile in ratio of 60: 40. The flow rate was adjusted at 1 mL/min and UV detection at 224 nm
  3. bThe values in the parenthesis are the corresponding theoretical values of t and F at P = 0.05
  4. *Units in (µg/band) for the proposed methods and in (µg/mL) for the reported one