A sustainable HPLC method coupled with diode array detection for versatile quantification of telmisartan, chlorthalidone and amlodipine in a fixed-dose antihypertensive formulation and dissolution studies

Table 4 Assessment of the proposed chromatographic method greenness according to Analytical Eco-Scale, GAPI and AGREEprep, blueness via BAGI tool and whiteness assessment by RGB 12 model

Eco-Scale assessment		GAPI Assessment^b	AGREEprep^c	BAGI Assessment^d	RGB12 algorithm^e
Reagents	Penalty points (PPs)
Acetonitrile	4
Potassium dihydrogen phosphate	4
Hydrochloric acid	4
Methanol	6
Instrument
Energy consumption^a	1
Occupational Hazard	0
Waste	6
Total PPs	25
Analytical Eco-Scale Total Score	75
Comment	Excellent green analysis

^aCalculated as: run time × flow rate for HPLC method
^bGAPI Assessment evaluated according to Green Analytical Procedure Index parameters description [21]
^cAGREEprep Assessment evaluated by using Analytical GREEnness Metric approach and software [24]
^dBlue Applicability Grade Index (BAGI) as a new metric tool for evaluating the practicality of an analytical method [29]
^eRGB12 algorithm for whiteness evaluation [27]

ISSN: 2661-801X