Table 1 System suitability parameters of the proposed HPLC–DAD method

Retention time (min ± 0.2)

4.3

5.3

8.5

Capacity Factor (k’)

1.05

1.52

3.05

Selectivity (α)*

1.45 2.01

 > 1

Resolution (R_s)^*

3.46 4.92

Rs > 2

Tailing factor (T)

1.15

1.21

1.24

T ≤ 2

Column efficiency (N)

5088

6875

6281

N ˃ 2000

Height equivalent to theoretical plate HETP  (cm/plate)

0.0049

0.0036

0.0040