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Table 1 Spectral features and validation parameters for the studied colorimetric approach

From: Full green assay of parenteral dosage forms of polymyxins utilizing xanthene dye: application to content uniformity testing

Parameters

Poly B

CS

λεξ (νµ)

545

Linear range (µg mL− 1)

1–9

1–6

Correlation coefficient (r)

0.9995

0.9995

Determination coefficient (r2)

0.999

0.9991

Intercept ± SD*

0.3882 ± 3.671 × 10− 3

0.3745 ± 2.449 × 10− 3

Slope ± SD

0.0552 ± 6.52 × 10− 4

0.0534 ± 6.21 × 10− 4

Sy.x**

0.005053

0.00294

***LOD (µg mL− 1)

0.22

0.15

****LOQ (µg mL− 1)

0.66

0.46

  1. The acceptability criteria for linearity is expressed as (r) and should be ≥ 0.9990
  2. *SD: Standard Deviation ** Sy.x: Standard Deviation of residuals from lines
  3. ***LOD: Limit of detection. ****LOQ: Limit of quantitation