Independent concentration extraction as a novel green approach resolving overlapped UV–Vis binary spectra and HPTLC-densitometric methods for concurrent determination of levocloperastine and chlorpheniramine

Table 2 Application of the proposed spectrophotometric and HPTLC-densitometric methods for the determination of the studied drugs in pure form

Drug	UV–Vis Spectrophotometry			TLC-densitometry			Reported method [16]
Drug	Conc. added (μg mL⁻¹)	Found^* (μg mL⁻¹)	%Recovery	Conc. added (μg/spot)	Found^* (μg/spot)	%Recovery	Taken (μg mL⁻¹)	Found^* (μg mL⁻¹)	%Recovery
CPM	6	5.96	99.33	0.3	0.30	102.40	10	10.04	100.44
	8	8.13	101.63	0.7	0.69	98.37	20	19.91	99.56
	12	11.98	99.83	1	1.01	100.91	30	30.04	100.15
	16	16.09	100.56	2	1.99	99.98
	22	21.91	99.59	3	2.98	99.48
	28	28.31	101.11	4	4.01	100.28
Mean% ± SD			100.34 ± 0.90			100.24 ± 1.36			100.05 ± 0.45
t-test	0.51 (2.36)			0.22 (2.36)
LCF	6	6.04	100.67	0.8	0.79	98.56	10	9.86	98.63
	8	7.93	99.13	2	2.01	100.25	20	20.27	101.36
	12	12.26	102.17	4	4.00	100.11	30	29.86	99.55
	16	15.89	99.31	6	5.97	99.46
	22	22.18	100.82	8	8.08	101.05
	28	27.87	99.54	10	9.95	99.50
Mean% ± SD			100.27 ± 1.16			99.82 ± 0.85			99.85 ± 1.39
t-test	0.40(2.36)			0.04 (2.36)

^* Comparison method involved HPLC determination of CPM and LCF using C18 column with mobile phase of buffer (pH 6.5) and acetonitrile (50:50, % v/v) as isocratic mobile phase, run time 8 min, flow rate 1 mL/min,linearity ranges 2–6 μg mL⁻¹ and 10–30 μg mL⁻¹ for CPM and LCF respectively and UV detection at 227 nm

ISSN: 2661-801X