Independent concentration extraction as a novel green approach resolving overlapped UV–Vis binary spectra and HPTLC-densitometric methods for concurrent determination of levocloperastine and chlorpheniramine

Table 1 Analytical performance data for the determination of the studied drugs by the proposed spectrophotometric and HPTLC-densitometric methods

Parameters	ICE	Z°E/US	TLC-densitometric
Parameters	CPM	LCF	CPM	LCF
Linearity range	3.0–30.0 μg mL⁻¹	3.0–30.0 μg mL⁻¹	0.3–4.0 μg/spot	0.8–10.0 μg/spot
Regression equation
Slope ± SD	1.2393 ± 0.00003	0.0397 ± 0.00019	3604.31 ± 13.95	3078.49 ± 20.19
Intercept ± confidence interval^*	0.0017 ± 0.0006	0.0076 ± 0.003	743.28 ± 26.334	122.46 ± 19.293
Correlation Coefficient (r)	0.999	0.999	0.999	0.999
LOD	0.13 μg mL⁻¹	0.66 μg mL⁻¹	0.03	0.13
LOQ	0.40 μg mL⁻¹	2.00 μg mL⁻¹	0.09 μg/spot	0.40 μg/spot

^* \(\overline{x}\pm t\frac{s}{\sqrt{n} }\) where \(\overline{x }\) is the mean value, and s is the standard deviation at n = 8 and the statistic value of t is 2.365 at 95% confidence interval with 7 degrees of freedom

ISSN: 2661-801X