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Table 3 Statistical comparison between results obtained by the proposed methods and the official methods [20,21,22]

From: Utilizing two different sustainable and green spectrophotometric approaches using derivative ratio spectra for the determination of a ternary severely overlapped mixture: application to veterinary formulation

Drug

TYL

SLD

TRI

Method

DD1

(TYL/SLD) (240 nm)

Approach 1

DD1

(TYL/TRI)

(254.7)

Approach 2

Official methoda

DD1

(272.2 nm)

Approach 1

DD1

(255.1 nm)

Approach 2

Official method b

DD1

(245 nm)

Approach 1

DDRD (236.5 nm)

Approach 2

Official methodc

Mean

100.51

99.82

100.45

99.93

100.23

100.06

100.04

99.88

100.09

S.D.

1.051

0.727

0.500

0.521

0.302

0.637

0.744

0.766

0.583

Variance

1.10400.

0.5285

0.2500

0.2714

0.0912

0.4057

0.5535

0.5867

0.3398

F test

(5.05)

4.416

2.112

–

1.498

4.448

–

1.628

1.726

–

Student's

t-test

(1.81)

0.13

1.7

–

0.401

0.587

–

0.110

0.514

–

  1. The figures in parenthesis are the corresponding theoretical values at P = 0.05
  2. The sample number(N) was 5 for all methods
  3. aBP Official method includes HPLC method using C18 column and sodium perchlorate (pH 2.5) adjusted with 1 M hydrochloric acid: acetonitrile (60,40, v/v) as mobile phase
  4. bBP Official method includes potentiometric nitrite titration
  5. cBP Official method includes HPLC method using methanol R and sodium perchlorate in ratios (30:70v/v) adjusted to pH 3.6 using phosphoric acid
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BMC Chemistry

ISSN: 2661-801X

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