Table 2 Summary of validation parameters for the reported RP-HPLC assay

Validation parameters

  Accuracy (mean ± SD)

99.97% ± 0.12

  Precision (%RSD)

Repeatability

0.86%

  Intraday and interday assay

Intraday: 0.33%

Interday: 0.60%

Linearity

  Regression equation

Y = 74.576x-17.466

  Regression coefficient (R²)

0.999

Sensitivity

  LOD

0.35 µg/mL

  LOQ

1.16 µg/mL

  Ruggedness (%RSD)

0.52%

Robustness

  %RSD for different pH

0.48%

  Flow rate

1.01%