Table 2 The constants of atropine, pirenzepine, darifenacin, and solifenacin with the immobilized M3R by the injection volume-dependent method

Atropine

250–1000

k′n_b/(1 + k′) = 2.42 × 10^–8–1.25 × 10^–5 k′Vm

0.993

1250–4000

k′n_b/(1 + k′) = 1.41 × 10^–7–1.02 × 10^–4 k′Vm

0.990

Pirenzepine

10–150

k′n_b/(1 + k′) = 3.75 × 10^–9–3.84 × 10^–6 k′Vm

0.986

300–1800

k′n_b/(1 + k′) = 3.14 × 10^–8–4.27 × 10^–5 k′Vm

0.987

Darifenacin

500–2000

k′n_b/(1 + k′) = 2.76 × 10^–8–1.29 × 10^–5 k′Vm

0.977

Solifenacin

200–1000

k′n_b/(1 + k′) = 4.40 × 10^–8–8.33 × 10^–6 k′Vm

0.976