Fig. 3

Identification of Halo-tagged M3R in Escherichia coli. a SDS-PAGE analysis of cell lysates. The circles indicate the location of the M3R; (b) Western blot analysis of M3R. Protein marker (M), 10 µL of Luria–Bertani medium (L), 10 µL of auto-induction medium (A), 10, 20, and 30 µL of cell lysate supernatant (S1, S2, S3), and 10 µL of cell lysate precipitate (P). Full-length gel and blots are presented in Additional file 1: Fig. S1