Fig. 11
Identification of M3R antagonists from Datura Flos using the immobilized M3R. a Shown here is a representative chromatogram of the Daturae Flos extract on the immobilized M3R column. Peak 2, characterized by a retention time exceeding the void time, is indicative of compounds within the extract that specifically interact with M3R. b The total ion current corresponding to peak 2 in the chromatogram was measured utilizing a reverse-phase XDB-C18 column (2.1 mm × 150 mm, 3.5 μm) in conjunction with an electrospray-MS/MS system. c Hyoscyamine was conclusively determined as compound 1 via HPLC–MS/MS analysis. d Scopolamine was established as compound 2 through HPLC–MS/MS analysis