Table 1 Different composition of mobile phases and the corresponding RF for the separation of DLX ‒ AVN binary mixture

Methanol-acetone (2:8, v/v)

0.1 with tailing

0.7 with tailing

Methanol-acetone (8:2, v/v)

0.15 with tailing

0.7

N-hexane-ethyl acetate (2:8, v/v)

At baseline

0.1 with tailing

N-hexane-ethyl acetate (8:2, v/v)

At baseline

At baseline

Toluene-methanol (7:3, v/v)

0.3 with tailing

0.6

Ethyl acetate-acetonitrile (8:2, v/v)

At baseline

At baseline

Ethyl acetate l-acetone-ammonia (8:1:1, v/v/v)

0.33

0.27

Acetone-methanol-ammonia (8:1:1, v/v/v)

0.8

0.9

Acetone-ethanol-ammonia (8:1:1, v/v/v)

0.85

At solvent front

Ethyl acetate-toluene-methanol (7:2:1, v/v/v)

At baseline

0.17

Toluene-ethyl acetate-acetic acid (6:2:2, v/v/v)

0.2

At baseline

Methanol-acetone-ammonia (8:2:0.05, v/v/v)

0.23

0.75