Various techniques for resolving overlapping ultraviolet spectra of combination pharmaceutical dosage forms containing hydroxychloroquine and paracetamol

Table 5 Analysis of the pharmaceutical preparation (Plaquenil® tablets) by applying the advanced absorption subtraction method, spectrum subtraction method, Zero Crossing derivative and Bivariate spectrophotometric methods

	Advanced absorption subtraction method				Spectrum subtraction method								Zero crossing derivative method
	PAR				HCQ				PAR				HCQ
			Recovery%				Recovery%				Recovery%				Recovery%
	Tablet Taken (µg/mL)	Standard Added (µg/mL)	Tablet	Added	Tablet Taken (µg/mL)	Standard Added (µg/mL)	Tablet	Added	Tablet Taken (µg/mL)	Standard Added (µg/mL)	Tablet	Added	Tablet Taken (µg/mL)	Standard Added (µg/mL)	Tablet	Added
	10	5	98.92	99.21	10	5	99.64	99.26	10	5	99.31	99.03	10	5	102.01	100.52
		10	101.81	98.76		10	101.19	98.03		10	100.66	100.64		10	101.65	99.80
		15	101.15	98.42		15	98.96	99.23		15	99.66	100.86		15	101.30	100.10
Mean			100.63	98.80			99.93	98.84			99.88	100.17			101.65	100.14
SD			1.51	0.40			1.14	0.70			0.70	1.00			0.36	0.36

	Zero Crossing derivative method				Bivariate method
	PAR				HCQ				PAR
			Recovery%		Recovery%						Recovery%
	Tablet Taken (µg/mL)	Standard Added (µg/mL)	Tablet	Added	Tablet Taken (µg/mL)	Standard Added (µg/mL)	Tablet	Added	Tablet Taken (µg/mL)	Standard Added (µg/mL)	Tablet	Added
	10	5	100.24	99.45	10	5	98.34	99.59	10	5	98.18	99.39
		10	99.32	101.17		10	98.15	99.74		10	98.43	100.38
		15	98.03	98.54		15	98.05	101.91		15	98.60	98.03
Mean			99.20	99.72			98.18	100.20			98.40	99.26
SD			1.11	1.33			0.15	1.03			0.21	1.18

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