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Fig. 9 | BMC Chemistry

Fig. 9

From: Homogeneous liquid–liquid microextraction coupled with HPLC/DAD for determination of nirmatrelvir and ritonavir as COVID-19 combination therapy in human plasma

Fig. 9

Chromatograms of human plasma containing 1 velpatasvir (IS, 20 µg/mL), 2 nirmatrelvir (15 µg/mL) and 3 ritonavir (15 µg/mL) after sample preparation by protein precipitation (red) and SULLME (black). Chromatographic conditions: Column: Thermo Hypersil ODS C8 column (250 × 4.6 mm, 5 μm) at 35 °C, Mobile phase: phosphate buffer (50 mM, pH = 3): acetonitrile (35:65, v/v), Elution: Isocratic, Detection: DAD at 210 nm, Flow rate: 1 mL/min, Injection volume: 5 µL

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BMC Chemistry

ISSN: 2661-801X

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