Figure 11

Effect of GO on mineralized matrix (MM) formation as a marker of osteoblastic differentiation, as detected by staining with Alizarin red S. The upper panel of 8 wells is a whole-plate photograph with two replicates of each condition. The lower panel shows pictures of MM formation in undifferentiated and differentiated control cells, and in cells pretreated with GO for 3 days (10× objective lens)