Figure 11

Analytical HPLC profiles of peptides obtained by in-water synthesis. a) Boc- Phe-Leu-NH2. b) Boc-Gly-Phe-Leu-NH2. c) Boc-Gly-Gly-Phe-Leu-NH2. d) Boc-Tyr(tBu)-Gly-Gly-Phe-Leu-NH2. Elution was carried out for over 30 min at a flow rate of 1 ml/min with a linear gradient from 9:1 to 3:7 mixture of 0.05% aqueous TFA and 0.05% TFA in acetonitrile.